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--- howto:how_to_measure_the_instrument_response_function_irf [2021/08/06 07:57] – buschmann
+++ howto:how_to_measure_the_instrument_response_function_irf [2023/02/23 07:05] – lan
@@ Line 11: / Line 11: @@
 ==== Make sure that the detection count rate is much lower than the count rate used for fluorescence decay measurement. ====
-Diluting the scattering solution is better than using grey filters. Ideal is when the decay and the IRF are recorded at the same [[glossary:differential count rate]] (and NOT at the same average count rates).
+**Note:** Diluting the scattering solution is better than using grey filters. Ideal is when the decay and the IRF are recorded at the same [[glossary:differential count rate]] (and NOT at the same average count rates).
 If the IRF should be measured on a microscope system with SPAD detectors, in the UV range also the Raman-scattering of water can be used. E.g. the Raman scattering can be recorded with a HQ480/40 bandpass filter, if a 405nm diode is used. This method is less suited for long wavelengths, as the Raman efficiency decreases. The water must be very pure in order not to capture any fluorescence.
@@ Line 43: / Line 43: @@
  prepare 1mL of saturated water solution of KI (potassium iodide)
  add 0.17 mL of saturated water solution of Erythrosine B (at least 95% of purity)
- add 0.03 mL of 0.004 M KOH (potassium hydroxide) solution in order to achieve pH10
 </code>
@@ Line 145: / Line 144: @@
 Picosecond fluorescence of intact and dissolved PSI-LHCI crystals\\
 Biophysical Journal, Vol.95, p.5851-5861 (2008)\\
-http://dx.doi.org/10.1529/biophysj.108.140467
+https://www.sciencedirect.com/science/article/pii/S0006349508820012