howto:how_to_measure_the_instrument_response_function_irf
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howto:how_to_measure_the_instrument_response_function_irf [2019/11/04 09:45] – [Appropriate Count Rate for Measuring an IRF] buschmann | howto:how_to_measure_the_instrument_response_function_irf [2023/09/07 22:54] – [How to Measure the Instrument Response Function (IRF)] peter | ||
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====== How to Measure the Instrument Response Function (IRF) ====== | ====== How to Measure the Instrument Response Function (IRF) ====== | ||
- | (For an explanation of the term IRF see [[glossary: | + | (For an explanation of the term Instrument Response Function |
+ | |||
+ | The following video shows how to measure na IRF on confocal microscopes. | ||
{{youtube> | {{youtube> | ||
+ | |||
+ | \\ | ||
- | ==== Make sure that the detection | + | ==== Low count rate during IRF measurements |
- | Diluting the scattering solution is better than using grey filters. Ideal is when the decay and the IRF are recorded at the same [[glossary: | + | Make sure that the detection count rate is much lower than the count rate during a fluorescence decay measurement. |
- | If the IRF should be measured on a microscope system with SPAD detectors, | + | If the IRF should be measured |
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add 0.17 mL of saturated water solution of Erythrosine B (at least 95% of purity) | add 0.17 mL of saturated water solution of Erythrosine B (at least 95% of purity) | ||
- | add 0.03 mL of 0.004 M KOH (potassium hydroxide) solution in order to achieve pH10 | ||
</ | </ | ||
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Evaluation of instrument response functions for lifetime imaging detectors using quenched Rose Bengal solutions\\ | Evaluation of instrument response functions for lifetime imaging detectors using quenched Rose Bengal solutions\\ | ||
Chemical Physics Letters, Vol.471, p.153-159 (2009)\\ | Chemical Physics Letters, Vol.471, p.153-159 (2009)\\ | ||
- | http://dx.doi.org/10.1016/j.cplett.2009.02.001 | + | https://www.sciencedirect.com/science/article/ |
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Collisional quenching of Erythrosine B as a potential reference dye for impulse response function evaluation\\ | Collisional quenching of Erythrosine B as a potential reference dye for impulse response function evaluation\\ | ||
Applied Spectroscopy, | Applied Spectroscopy, | ||
- | http://www.ingentaconnect.com/content/sas/ | + | https://www.osapublishing.org/as/viewmedia.cfm? |
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Photophysical properties of novel fluorescein derivative and its applications for time-resolved fluorescence spectroscopy\\ | Photophysical properties of novel fluorescein derivative and its applications for time-resolved fluorescence spectroscopy\\ | ||
Chemical Physics Letters, Vol.493, p.399-403 (2010)\\ | Chemical Physics Letters, Vol.493, p.399-403 (2010)\\ | ||
- | http://dx.doi.org/10.1016/j.cplett.2010.05.061 | + | https://www.sciencedirect.com/science/article/ |
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Picosecond fluorescence of intact and dissolved PSI-LHCI crystals\\ | Picosecond fluorescence of intact and dissolved PSI-LHCI crystals\\ | ||
Biophysical Journal, Vol.95, p.5851-5861 (2008)\\ | Biophysical Journal, Vol.95, p.5851-5861 (2008)\\ | ||
- | http://dx.doi.org/10.1529/biophysj.108.140467 | + | https://www.sciencedirect.com/science/article/ |
howto/how_to_measure_the_instrument_response_function_irf.txt · Last modified: 2023/09/07 22:55 by peter