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--- howto:how_to_measure_the_instrument_response_function_irf [2023/09/07 22:45] – [How to Measure the Instrument Response Function (IRF)] peter
+++ howto:how_to_measure_the_instrument_response_function_irf [2023/09/07 22:54] – [How to Measure the Instrument Response Function (IRF)] peter
@@ Line 5: / Line 5: @@
 ====== How to Measure the Instrument Response Function (IRF) ======
 (For an explanation of the term Instrument Response Function see [[glossary:IRF]])
+The following video shows how to measure na IRF on confocal microscopes.
 {{youtube>cjSgCBe3ReM?large}}
+\\
-==== Make sure that the detection count rate is much lower than the count rate used for fluorescence decay measurement. ====
+==== Low count rate during IRF measurements is important! ====
-**Note:** Diluting the scattering solution is better than using grey filters. Ideal is when the decay and the IRF are recorded at the same [[glossary:differential count rate]] (and NOT at the same average count rates).
+Make sure that the detection count rate is much lower than the count rate during a fluorescence decay measurement. Diluting the scattering solution is better than using grey (ND) filters. Ideal is when the decay and the IRF are recorded at the same [[glossary:differential count rate]] (and NOT at the same average count rate).
-If the IRF should be measured on a microscope system with SPAD detectors, in the UV range also the Raman-scattering of water can be used. E.g. the Raman scattering can be recorded with a HQ480/40 bandpass filter, if a 405nm diode is used. This method is less suited for long wavelengths, as the Raman efficiency decreases. The water must be very pure in order not to capture any fluorescence.
+If the IRF should be measured in the UV range on a microscope system with SPAD detectors, the Raman scattering of water can be used, too. E.g. the Raman scattering can be recorded with a HQ480/40 bandpass filter, if a 405nm laser diode is used. This method is less suited for long wavelengths, as the Raman scattering decreases. In order to avoid signal contamination by any fluorescence, the water must be very pure.