howto:how_to_measure_the_instrument_response_function_irf
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howto:how_to_measure_the_instrument_response_function_irf [2016/12/06 12:05] – veiga | howto:how_to_measure_the_instrument_response_function_irf [2021/08/06 07:57] – buschmann | ||
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===== Appropriate Count Rate for Measuring an IRF ===== | ===== Appropriate Count Rate for Measuring an IRF ===== | ||
See [[glossary: | See [[glossary: | ||
- | + | ||
+ | ===== How often does the IRF need to be measured? ===== | ||
+ | |||
+ | In spectrometers, | ||
+ | |||
+ | In microscopy-applications, | ||
+ | If the intensity needs to be changed, the optical attenuation can be adapted. | ||
+ | |||
+ | A special case are systems with 2-Photon-Excitation (2PE). Here, usually TiSa-lasers are used which have fs-pulses, therefore the IRF is normally determined by the detector. In these cases, often the IRF can be measured once (and the excitation wavelength is not important, provided that the IRF is measured with a quenched dye and the same filterset is used as for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the position of the IRF can shift slightly, but this is accounted for with the " | ||
+ | |||
===== How to compensate IRF effects in the analysis of time domain measurements ===== | ===== How to compensate IRF effects in the analysis of time domain measurements ===== | ||
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Evaluation of instrument response functions for lifetime imaging detectors using quenched Rose Bengal solutions\\ | Evaluation of instrument response functions for lifetime imaging detectors using quenched Rose Bengal solutions\\ | ||
Chemical Physics Letters, Vol.471, p.153-159 (2009)\\ | Chemical Physics Letters, Vol.471, p.153-159 (2009)\\ | ||
- | http://dx.doi.org/10.1016/j.cplett.2009.02.001 | + | https://www.sciencedirect.com/science/article/ |
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Collisional quenching of Erythrosine B as a potential reference dye for impulse response function evaluation\\ | Collisional quenching of Erythrosine B as a potential reference dye for impulse response function evaluation\\ | ||
Applied Spectroscopy, | Applied Spectroscopy, | ||
- | http://www.ingentaconnect.com/content/sas/ | + | https://www.osapublishing.org/as/viewmedia.cfm? |
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Photophysical properties of novel fluorescein derivative and its applications for time-resolved fluorescence spectroscopy\\ | Photophysical properties of novel fluorescein derivative and its applications for time-resolved fluorescence spectroscopy\\ | ||
Chemical Physics Letters, Vol.493, p.399-403 (2010)\\ | Chemical Physics Letters, Vol.493, p.399-403 (2010)\\ | ||
- | http://dx.doi.org/10.1016/j.cplett.2010.05.061 | + | https://www.sciencedirect.com/science/article/ |
howto/how_to_measure_the_instrument_response_function_irf.txt · Last modified: 2023/09/07 22:55 by peter