howto:how_to_measure_the_instrument_response_function_irf
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howto:how_to_measure_the_instrument_response_function_irf [2015/06/03 14:58] – admin | howto:how_to_measure_the_instrument_response_function_irf [2016/10/18 11:40] – [Selected literature:] buschmann | ||
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+ | The dye **Allura Red** has also a remarkably short fluorescence lifetime of about 10 ps. This short lifetime does not depend on the emission (observation) wavelength (from 550 nm to 750 nm) and it is another candidate for impulse response measurements. | ||
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+ | Allura Red is Disodium 6-hydroxy-5-[(2-methoxy-5-methyl-4-sulfophenyl)azo]-2-naphthalenesulfonate, | ||
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+ | Rahul Chib, Sunil Shah, Zygmunt Gryczynski, Rafal Fudala, Julian Borejdo, Bogumil Zelent, Maria G Corradini, Richard D Ludescher, Ignacy Gryczynski\\ | ||
+ | Standard reference for instrument response function in fluorescence lifetime measurements in visible and near infrared\\ | ||
+ | Measurement Science and Technology, Vol.27, 027001 (2016)\\ | ||
+ | http:// | ||
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+ | Pyridinium Styryl Dyes have also been used as IRF standard: | ||
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+ | Lei Li, Mengfang Chang, Hua Yi, Menghui Jia, Siaodan Cao, Zhongneng Zhou, Sanjun Zhang, Haifeng Pan, Chun-Wei Shih, Ralph Jimenez, Jianhua Xu\\ | ||
+ | Using Pyridinium Styryl Dyes as the Standards of Time-Resolved Instrument Response\\ | ||
+ | Applied Spectroscopy, | ||
+ | http:// | ||
Even dye with lifetimes that are usually in the ns range can be used, if the lifetime can be quenched to a few ps. Quenching can be achieved using a saturated iodide solution. For details see: | Even dye with lifetimes that are usually in the ns range can be used, if the lifetime can be quenched to a few ps. Quenching can be achieved using a saturated iodide solution. For details see: | ||
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===== Appropriate Count Rate for Measuring an IRF ===== | ===== Appropriate Count Rate for Measuring an IRF ===== | ||
- | [[glossary:Differential_Count_Rate]] | + | See [[glossary:Differential Count Rate]] |
===== How to compensate IRF effects in the analysis of time domain measurements ===== | ===== How to compensate IRF effects in the analysis of time domain measurements ===== |
howto/how_to_measure_the_instrument_response_function_irf.txt · Last modified: 2023/09/07 22:55 by peter