FLIM Measurement Using a Nikon A1 with a FLIM and FCS Upgrade

This tutorial shows the recording of FLIM images using an LSM upgrade kit, in this case a Nikon A1. In FLIM-measurements, the fluorescence lifetime of a component can be determined which can be used e.g.

to distinguish different tissue or cell constituents

to monitor concentration changes using a reporter dye

To monitor interactions between two molecules via FRET. Recording of FLIM images especially for

FLIM-FRET is also shown in a separate video (see link below).

The general principles of the data acquisition are analogous in all LSM upgrade kits. The movie was recorded in 02/2014; the recording procedure may be somewhat different in older or newer models.

Update: NIS Elements 4.3 integrates control for FLIM and FCS

The movie was recorded in 02/2015; the recording procedure may be somewhat different in older or newer models.

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