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--- howto:flim_fret_calculation_for_multi_exponential_donors [2014/06/18 10:39] – admin
+++ howto:flim_fret_calculation_for_multi_exponential_donors [2020/05/12 12:37] – [Step-by-Step Tutorial] ruckelshausen
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 {{tag>tutorial howto analysis FLIM imaging FRET SymPhoTime}}
-~~TOC~~
+~~NOTOC~~
 ====== FLIM FRET Calculation for Multi Exponential Donors ======
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 ===== Step-by-Step Tutorial =====
+**Note:** This Step-by-Step tutorial was written for SymPhoTime version 2.5. There might be slight differences compared to other software versions. For example the way of setting an intensity threshold has changed. In the video the former version is shown.
 ==== Determine the donor only lifetime using the FLIM script ====