howto:flim_fret_calculation_for_multi_exponential_donors
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howto:flim_fret_calculation_for_multi_exponential_donors [2014/05/23 07:43] – admin | howto:flim_fret_calculation_for_multi_exponential_donors [2014/06/18 10:39] – admin | ||
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This tutorial shows step-by-step, | This tutorial shows step-by-step, | ||
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+ | ===== Background information ===== | ||
FLIM-FRET is a robust method to determine the FRET efficiency of a suited donor acceptor pair. | FLIM-FRET is a robust method to determine the FRET efficiency of a suited donor acceptor pair. | ||
If the FRET-donor molecule has a more exponential decay kinetic, the amplitude weighted average lifetime has to be used to calculate the average FRET efficiency. | If the FRET-donor molecule has a more exponential decay kinetic, the amplitude weighted average lifetime has to be used to calculate the average FRET efficiency. | ||
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* To analyze many images with the same FRET pair, it is possible to save the starting values for the fits by clicking on "Save Defaults" | * To analyze many images with the same FRET pair, it is possible to save the starting values for the fits by clicking on "Save Defaults" | ||
* If you don't want to do anything more, just close the result window. | * If you don't want to do anything more, just close the result window. | ||
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howto/flim_fret_calculation_for_multi_exponential_donors.txt · Last modified: 2023/11/21 10:33 by lan