howto:flim_fret_calculation_for_multi_exponential_donors
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howto:flim_fret_calculation_for_multi_exponential_donors [2014/02/21 08:57] – rosario | howto:flim_fret_calculation_for_multi_exponential_donors [2014/05/23 07:43] – admin | ||
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====== FLIM FRET Calculation for Multi Exponential Donors ====== | ====== FLIM FRET Calculation for Multi Exponential Donors ====== | ||
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**Note:**\\ | **Note:**\\ | ||
- | For more information about the different ROI selection tools, check the tutorial | + | For more information about the different ROI selection tools, check the tutorial [[howto: |
* Draw a ROI around one of the left cells by maintaining the left mouse button activated while circling the selected part of the image. Then release the left mouse button, press " | * Draw a ROI around one of the left cells by maintaining the left mouse button activated while circling the selected part of the image. Then release the left mouse button, press " | ||
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* To analyze many images with the same FRET pair, it is possible to save the starting values for the fits by clicking on "Save Defaults" | * To analyze many images with the same FRET pair, it is possible to save the starting values for the fits by clicking on "Save Defaults" | ||
* If you don't want to do anything more, just close the result window. | * If you don't want to do anything more, just close the result window. | ||
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howto/flim_fret_calculation_for_multi_exponential_donors.txt · Last modified: 2023/11/21 10:33 by lan