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--- howto:flim_fret_calculation_for_multi_exponential_donors [2014/02/21 08:57] – rosario
+++ howto:flim_fret_calculation_for_multi_exponential_donors [2014/02/21 10:12] – admin
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+{{tag>tutorial howto analysis FLIM imaging FRET}}
+~~TOC~~
 ====== FLIM FRET Calculation for Multi Exponential Donors ======
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 **Note:**\\
-For more information about the different ROI selection tools, check the tutorial "[[howto:roi_fitting_using_the_flim_script|ROI fitting using the FLIM script]]".
+For more information about the different ROI selection tools, check the tutorial [[howto:roi_fitting_using_the_flim_script|ROI fitting using the FLIM script]].
   * Draw a ROI around one of the left cells by maintaining the left mouse button activated while circling the selected part of the image. Then release the left mouse button, press "Shift" continuously and cycle the other left nucleus. This way, the donor only cells are marked as ROI 0.
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     * To analyze many images with the same FRET pair, it is possible to save the starting values for the fits by clicking on "Save Defaults" and store the starting values as a User Profile by entering the main menu ("Settings\Save User Profile as"). The software will need to restart to adapt the changes.
   * If you don't want to do anything more, just close the result window.