Time-Resolved Fluorescence Wiki

User Tools

Site Tools

Trace:
howto:flim_fret_calculation_for_multi_exponential_donors

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revisionPrevious revision
Next revisionBoth sides next revision
howto:flim_fret_calculation_for_multi_exponential_donors [2014/02/21 08:57] – rosariohowto:flim_fret_calculation_for_multi_exponential_donors [2014/02/21 09:19] – rosario
Line 77: Line 77:
  
 **Note:**\\ **Note:**\\
-For more information about the different ROI selection tools, check the tutorial "[[howto:roi_fitting_using_the_flim_script|ROI fitting using the FLIM script]]".+For more information about the different ROI selection tools, check the tutorial [[howto:roi_fitting_using_the_flim_script|ROI fitting using the FLIM script]].
  
   * Draw a ROI around one of the left cells by maintaining the left mouse button activated while circling the selected part of the image. Then release the left mouse button, press "Shift" continuously and cycle the other left nucleus. This way, the donor only cells are marked as ROI 0.   * Draw a ROI around one of the left cells by maintaining the left mouse button activated while circling the selected part of the image. Then release the left mouse button, press "Shift" continuously and cycle the other left nucleus. This way, the donor only cells are marked as ROI 0.
howto/flim_fret_calculation_for_multi_exponential_donors.txt · Last modified: 2023/11/21 10:33 by lan