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--- howto:flim-fret_calculation_for_single_exponential_donors [2020/05/05 16:06] – [Step-by-Step Tutorial] ruckelshausen
+++ howto:flim-fret_calculation_for_single_exponential_donors [2020/05/06 09:38] (current) – [Step-by-Step Tutorial] ruckelshausen
@@ Line 81: / Line 81: @@
   * In this example set the lower intensity threshold to 500 counts.
+{{ :howto:flim-fret-1expd_lowerthresh_500_bearb.png?400 |}}
   * Click "OK"
 **Response:** In the image, only pixels with higher photon counts are highlighted. Adapt the intensity maximum to see the highlighted pixels more clearly.
-{{ :howto:flim-fret-1expd_roi_from_thresh_2_v2.png?400 |}}
+{{ :howto:flim-fret-1expd_roi_from_thresh_2_v3.png?400 |}}
   * In the lifetime fitting panel, keep the default Fitting model "Mono-Exp. Donor".