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howto:determination_of_the_focal_width_with_the_focal

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howto:determination_of_the_focal_width_with_the_focal [2014/05/07 16:13] – adminhowto:determination_of_the_focal_width_with_the_focal [2014/06/18 11:00] – admin
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 This tutorial shows step-by-step, how to determine the Point Spread Function (PSF) of a microscopic system using the Focal Width Script. It requires the image of a subresolution bead, in which the center is marked in the image. The script applies then a Gaussian fit of the bead and calculates the Full Width Half Maximum (FWHM) in both scanning directions. This tutorial shows step-by-step, how to determine the Point Spread Function (PSF) of a microscopic system using the Focal Width Script. It requires the image of a subresolution bead, in which the center is marked in the image. The script applies then a Gaussian fit of the bead and calculates the Full Width Half Maximum (FWHM) in both scanning directions.
  
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 ===== Step-by-Step Tutorial ===== ===== Step-by-Step Tutorial =====
  
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   * Optional: Apply the same script to the file ''TS-bead_immo_xz-scan.ptu'' to get an idea about the ratio of the focal width in xy vs. z.   * Optional: Apply the same script to the file ''TS-bead_immo_xz-scan.ptu'' to get an idea about the ratio of the focal width in xy vs. z.
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howto/determination_of_the_focal_width_with_the_focal.txt · Last modified: 2015/01/22 14:48 by admin