howto:calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script
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| howto:calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script [2014/06/18 09:33] – admin | howto:calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script [2015/11/03 14:18] – admin | ||
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| The diffusion coefficient of ATTO488 carboxilic acid has been measured to be 400 µm²/s at 25°C. Published diffusion coefficients of various fluorophores are summarized in the Technical Note " | The diffusion coefficient of ATTO488 carboxilic acid has been measured to be 400 µm²/s at 25°C. Published diffusion coefficients of various fluorophores are summarized in the Technical Note " | ||
| Note that the diffusion coefficients of all dyes are temperature dependent.\\ | Note that the diffusion coefficients of all dyes are temperature dependent.\\ | ||
| - | FCS measurements are usually point measurements acquired with very sensitive detectors ([[glossary: | + | FCS measurements are usually point measurements acquired with very sensitive detectors ([[glossary: |
| FCS is a technique applicable over a wide range of concentrations, | FCS is a technique applicable over a wide range of concentrations, | ||
howto/calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script.txt · Last modified: 2019/02/07 12:16 by admin