howto:calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
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howto:calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script [2014/06/18 15:58] – admin | howto:calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script [2014/06/18 16:06] – admin | ||
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{{tag> tutorial howto FRET PIE analysis time-trace SymPhoTime}} | {{tag> tutorial howto FRET PIE analysis time-trace SymPhoTime}} | ||
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====== Calculate Ratiometric Single Pair FRET Distributions Using PIE-FRET ====== | ====== Calculate Ratiometric Single Pair FRET Distributions Using PIE-FRET ====== | ||
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This tutorial shows step-by-step, | This tutorial shows step-by-step, | ||
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**Note:** The script requires a time trace containing the fluorescence of two spectrally separated channels, one channel mainly detecting the fluorescence of the donor dye and the other channel detecting mainly the fluorescence of the acceptor dye.\\ | **Note:** The script requires a time trace containing the fluorescence of two spectrally separated channels, one channel mainly detecting the fluorescence of the donor dye and the other channel detecting mainly the fluorescence of the acceptor dye.\\ | ||
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**Note:** Further information about the sample used in this measurement can be found under: J.L. Fiore et. al., Enthalpy-driven RNA-folding: | **Note:** Further information about the sample used in this measurement can be found under: J.L. Fiore et. al., Enthalpy-driven RNA-folding: | ||
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howto/calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script.txt · Last modified: 2015/11/03 14:12 by admin