howto:calculate_ratiometric_single_pair_fret_distributions
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howto:calculate_ratiometric_single_pair_fret_distributions [2014/06/18 15:55] – admin | howto:calculate_ratiometric_single_pair_fret_distributions [2015/11/03 14:17] (current) – admin | ||
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{{tag> tutorial howto time-trace analysis FRET SymPhoTime}} | {{tag> tutorial howto time-trace analysis FRET SymPhoTime}} | ||
- | ~~TOC~~ | + | ~~NOTOC~~ |
====== Calculate Ratiometric Single Pair FRET Distributions ====== | ====== Calculate Ratiometric Single Pair FRET Distributions ====== | ||
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**Note:** The script requires a time trace containing the fluorescence of two spectrally separated channels, one channel mainly detecting the fluorescence of the donor dye and the other channel detecting mainly the fluorescence of the acceptor dye.\\ | **Note:** The script requires a time trace containing the fluorescence of two spectrally separated channels, one channel mainly detecting the fluorescence of the donor dye and the other channel detecting mainly the fluorescence of the acceptor dye.\\ | ||
The time traces can be acquired from single molecule events in a diluted solution, where the passages of single molecules are registered as " | The time traces can be acquired from single molecule events in a diluted solution, where the passages of single molecules are registered as " | ||
- | To record such traces, usually single molecule sensitive detectors as SPAD or Hybrid | + | To record such traces, usually single molecule sensitive detectors as [[glossary:SPAD]] or [[glossary:Hybrid |
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howto/calculate_ratiometric_single_pair_fret_distributions.txt · Last modified: 2015/11/03 14:17 by admin