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--- howto:calculate_ratiometric_single_pair_fret_distributions [2014/06/18 15:48] – admin
+++ howto:calculate_ratiometric_single_pair_fret_distributions [2015/11/03 14:17] (current) – admin
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 {{tag> tutorial howto time-trace analysis FRET SymPhoTime}}
-~~TOC~~
+~~NOTOC~~
 ====== Calculate Ratiometric Single Pair FRET Distributions ======
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 This tutorial shows step-by-step, how to calculate a [[glossary:fret|FRET]] histogram from single FRET pairs detected under single molecule conditions. The script requires a spectrally resolved fluorescence time trace. As a result, the distribution of FRET efficiencies of different molecules is shown which can be used to detect subpopulations.
+{{ youtube>YS_z5uA2PV4?large }}
 ===== Prerequisites =====
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 **Note:** The script requires a time trace containing the fluorescence of two spectrally separated channels, one channel mainly detecting the fluorescence of the donor dye and the other channel detecting mainly the fluorescence of the acceptor dye.\\
 The time traces can be acquired from single molecule events in a diluted solution, where the passages of single molecules are registered as "bursts". Alternatively, traces obtained from a stationary single FRET pair can be analyzed, e.g. to observe conformational changes.\\
-To record such traces, usually single molecule sensitive detectors as SPAD or Hybrid PMA are necessary to successfully detect single molecule fluorescence.\\
+To record such traces, usually single molecule sensitive detectors as [[glossary:SPAD]] or [[glossary:Hybrid PMT]]s are necessary to successfully detect single molecule fluorescence.\\
  \\