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howto:calculate_ratiometric_single_pair_fret_distributions [2014/06/18 15:48] – adminhowto:calculate_ratiometric_single_pair_fret_distributions [2014/06/18 15:56] – admin
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 {{tag> tutorial howto time-trace analysis FRET SymPhoTime}} {{tag> tutorial howto time-trace analysis FRET SymPhoTime}}
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 ====== Calculate Ratiometric Single Pair FRET Distributions ====== ====== Calculate Ratiometric Single Pair FRET Distributions ======
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 This tutorial shows step-by-step, how to calculate a [[glossary:fret|FRET]] histogram from single FRET pairs detected under single molecule conditions. The script requires a spectrally resolved fluorescence time trace. As a result, the distribution of FRET efficiencies of different molecules is shown which can be used to detect subpopulations.  This tutorial shows step-by-step, how to calculate a [[glossary:fret|FRET]] histogram from single FRET pairs detected under single molecule conditions. The script requires a spectrally resolved fluorescence time trace. As a result, the distribution of FRET efficiencies of different molecules is shown which can be used to detect subpopulations. 
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 ===== Prerequisites ===== ===== Prerequisites =====
howto/calculate_ratiometric_single_pair_fret_distributions.txt · Last modified: 2015/11/03 14:17 by admin