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howto:calculate_ratiometric_fret-images

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howto:calculate_ratiometric_fret-images [2014/06/18 09:20] – adminhowto:calculate_ratiometric_fret-images [2014/06/18 09:24] – admin
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 This tutorial shows step-by-step, how the "FRET Image" script of [[products:SymPhoTime64|SymPhoTime 64]] can be used to calculate pixel-by-pixel the average [[glossary:fret|FRET]] efficiency in an image containing several cells transfected with Cerulean/YFP constructs. To calculate the average FRET efficiency, a ratiometric approach based on the intensities in the donor and the acceptor channel is used. This tutorial shows step-by-step, how the "FRET Image" script of [[products:SymPhoTime64|SymPhoTime 64]] can be used to calculate pixel-by-pixel the average [[glossary:fret|FRET]] efficiency in an image containing several cells transfected with Cerulean/YFP constructs. To calculate the average FRET efficiency, a ratiometric approach based on the intensities in the donor and the acceptor channel is used.
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 ===== Background Information ===== ===== Background Information =====
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     * In case one wants to analyze many images with the same FRET pair, it is possible to save the starting values for the fits by clicking on "Save Defaults" and store the starting values as a User Profile by entering the main menu ("Settings\Save User Profile as"). The software will need to restart to adapt the changes.     * In case one wants to analyze many images with the same FRET pair, it is possible to save the starting values for the fits by clicking on "Save Defaults" and store the starting values as a User Profile by entering the main menu ("Settings\Save User Profile as"). The software will need to restart to adapt the changes.
   * If you don't want to do anything more, just close the result window.   * If you don't want to do anything more, just close the result window.
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howto/calculate_ratiometric_fret-images.txt · Last modified: 2015/02/25 14:00 by bornemann