howto:calculate_fccs_trace_with_the_grouped_fcs_script
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| howto:calculate_fccs_trace_with_the_grouped_fcs_script [2014/06/18 08:42] – admin | howto:calculate_fccs_trace_with_the_grouped_fcs_script [2015/02/25 14:01] – bornemann | ||
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| - | ====== Calculate | + | ====== Calculate |
| ===== Summary ===== | ===== Summary ===== | ||
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| ===== Background Information ===== | ===== Background Information ===== | ||
| - | **Note: | + | Fluorescence Cross-Correlation Spectroscopy (FCCS) is a method to determine molecular interactions between two molecules labeled with different dyes. Usually, the dyes attached to the two molecules of interest differ in their absorption and emission properties. They can also vary in their fluorescence lifetime, for more information please check the tutorials for Fluorescence Lifetime Correlation Spectroscopy (FLCS).\\ |
| To quantify the cross-correlation signal and connect it to a binding fraction, a calibration of the effective confocal volumes for each dye and the overlapping confocal volume must be performed.\\ | To quantify the cross-correlation signal and connect it to a binding fraction, a calibration of the effective confocal volumes for each dye and the overlapping confocal volume must be performed.\\ | ||
| The calibration can be done using a molecule labeled with both fluorophores.\\ | The calibration can be done using a molecule labeled with both fluorophores.\\ | ||
howto/calculate_fccs_trace_with_the_grouped_fcs_script.txt · Last modified: 2020/04/01 15:05 by buschmann