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howto:calculate_fccs_trace_with_the_grouped_fcs_script

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howto:calculate_fccs_trace_with_the_grouped_fcs_script [2014/05/23 07:44] adminhowto:calculate_fccs_trace_with_the_grouped_fcs_script [2014/06/18 08:42] admin
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 {{tag> analysis howto tutorial FCS FCCS Cross-Correlation  SymPhoTime}} {{tag> analysis howto tutorial FCS FCCS Cross-Correlation  SymPhoTime}}
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-====== Calculate FCCS Traces with the Grouped FCS Script ======+====== Calculate multiple FCCS Traces with the Grouped FCS Script ======
  
 ===== Summary ===== ===== Summary =====
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 This tutorial shows step-by-step, how to calculate a spectral cross-correlation curve and the corresponding autocorrelation curves of a dye-labeled DNA-oligonucleotide using the Grouped FCS script. This tutorial shows step-by-step, how to calculate a spectral cross-correlation curve and the corresponding autocorrelation curves of a dye-labeled DNA-oligonucleotide using the Grouped FCS script.
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 ===== Background Information ===== ===== Background Information =====
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 **Note:** In a sample as measured here by a combination of pulsed and cw excitation, it is possible to remove the false positive cross-correlation, which significantly simplifies the binding analysis. This is explained in the tutorial [[howto:using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs|Using the FLCS script for spectral crosstalk removal via FLCCS]]. **Note:** In a sample as measured here by a combination of pulsed and cw excitation, it is possible to remove the false positive cross-correlation, which significantly simplifies the binding analysis. This is explained in the tutorial [[howto:using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs|Using the FLCS script for spectral crosstalk removal via FLCCS]].
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howto/calculate_fccs_trace_with_the_grouped_fcs_script.txt · Last modified: 2020/04/01 15:05 by buschmann