howto:calculate_fccs_trace_with_the_grouped_fcs_script
Differences
This shows you the differences between two versions of the page.
Both sides previous revisionPrevious revisionNext revision | Previous revisionNext revisionBoth sides next revision | ||
howto:calculate_fccs_trace_with_the_grouped_fcs_script [2014/05/23 07:44] – admin | howto:calculate_fccs_trace_with_the_grouped_fcs_script [2014/06/18 09:21] – admin | ||
---|---|---|---|
Line 1: | Line 1: | ||
- | {{tag> analysis howto tutorial FCS FCCS Crosscorrelation | + | {{tag> analysis howto tutorial FCS FCCS Cross-Correlation |
- | ~~TOC~~ | + | ~~NOTOC~~ |
- | ====== Calculate FCCS Traces with the Grouped FCS Script ====== | + | ====== Calculate |
===== Summary ===== | ===== Summary ===== | ||
Line 8: | Line 8: | ||
This tutorial shows step-by-step, | This tutorial shows step-by-step, | ||
+ | |||
+ | {{ youtube> | ||
===== Background Information ===== | ===== Background Information ===== | ||
- | **Note: | + | Fluorescence Cross-Correlation Spectroscopy (FCCS) is a method to determine molecular interactions between two molecules labeled with different dyes. Usually, the dyes attached to the two molecules of interest differ in their absorption and emission properties. They can also vary in their fluorescence lifetime, for more information please check the tutorials for Fluorescence Lifetime Correlation Spectroscopy (FLCS).\\ |
To quantify the cross-correlation signal and connect it to a binding fraction, a calibration of the effective confocal volumes for each dye and the overlapping confocal volume must be performed.\\ | To quantify the cross-correlation signal and connect it to a binding fraction, a calibration of the effective confocal volumes for each dye and the overlapping confocal volume must be performed.\\ | ||
The calibration can be done using a molecule labeled with both fluorophores.\\ | The calibration can be done using a molecule labeled with both fluorophores.\\ | ||
Line 101: | Line 103: | ||
**Note:** In a sample as measured here by a combination of pulsed and cw excitation, it is possible to remove the false positive cross-correlation, | **Note:** In a sample as measured here by a combination of pulsed and cw excitation, it is possible to remove the false positive cross-correlation, | ||
- |
howto/calculate_fccs_trace_with_the_grouped_fcs_script.txt · Last modified: 2020/04/01 15:05 by buschmann