howto:calculate_and_fit_fcs_traces_with_the_fcs_script
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| howto:calculate_and_fit_fcs_traces_with_the_fcs_script [2014/06/18 08:36] – admin | howto:calculate_and_fit_fcs_traces_with_the_fcs_script [2014/06/18 09:21] – admin | ||
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| ===== Background Information ===== | ===== Background Information ===== | ||
| - | **Note: | + | ATTO488 (ATTO-Tec, Germany) is a suited calibration dye to calibrate the confocal microscope at ~470 – 490 nm excitation and detection ~500 – 540 nm.\\ |
| The diffusion coefficient of ATTO488 carboxilic acid has been measured to be 400 µm²/s at 25°C. Published diffusion coefficients of various fluorophores are summarized in the Technical Note " | The diffusion coefficient of ATTO488 carboxilic acid has been measured to be 400 µm²/s at 25°C. Published diffusion coefficients of various fluorophores are summarized in the Technical Note " | ||
| Note that the diffusion coefficients of all dyes are temperature dependent.\\ | Note that the diffusion coefficients of all dyes are temperature dependent.\\ | ||
howto/calculate_and_fit_fcs_traces_with_the_fcs_script.txt · Last modified: 2015/11/03 14:11 by admin