howto:avoid_pile_up_effect_in_flim_measurements
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howto:avoid_pile_up_effect_in_flim_measurements [2015/03/26 12:09] – admin | howto:avoid_pile_up_effect_in_flim_measurements [2015/06/03 15:11] (current) – admin | ||
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{{tag> howto FLIM acquisition pile-up TCSPC}} | {{tag> howto FLIM acquisition pile-up TCSPC}} | ||
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+ | ~~TOC~~ | ||
====== How to Avoid the Pile-up Effect in FLIM Measurements ====== | ====== How to Avoid the Pile-up Effect in FLIM Measurements ====== | ||
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* Determine the number of photons via the data reader (right mouse click) in a very bright pixel within the FastFLIM image | * Determine the number of photons via the data reader (right mouse click) in a very bright pixel within the FastFLIM image | ||
* Calculate the count rate for this bright pixel: Divide the number of photons per pixel by the exposure time per pixel | * Calculate the count rate for this bright pixel: Divide the number of photons per pixel by the exposure time per pixel | ||
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+ | ===== Differential Count Rate ===== | ||
+ | see [[glossary: |
howto/avoid_pile_up_effect_in_flim_measurements.txt · Last modified: 2015/06/03 15:11 by admin