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--- howto:avoid_pile_up_effect_in_flim_measurements [2015/03/26 12:08] – [Measurement of Pile Up Limit] admin
+++ howto:avoid_pile_up_effect_in_flim_measurements [2015/06/03 15:11] (current) – admin
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 {{tag> howto FLIM acquisition pile-up TCSPC}}
+~~TOC~~
 ====== How to Avoid the Pile-up Effect in FLIM Measurements ======
@@ Line 22: / Line 24: @@
-===== Measurement of Pile Up Limit =====
+===== Measurement of the Pile Up Limit =====
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   * At a pixel dwell time of 10µs each pixel is displayed as one point in the time trace. At a pixel dwell time of 1 µs the average of 10 pixels are displayed as one point in the time trace.
   * During the Test FLIM measurement the laser intensity is set that the time trace is only at rare instances hitting the red line.
+===== Calculation of the count rate per pixel to determine the Pile-Up effect  =====
+For an already acquired FLIM measurement it is possible to determine the count rate per pixel to determine if a pile-up effect is present.
+Procedure:
+  * Calculate the exposure time per pixel: Multiply the number of frames with the pixel dwell time
+  * Determine the number of photons via the data reader (right mouse click) in a very bright pixel within the FastFLIM image
+  * Calculate the count rate for this bright pixel: Divide the number of photons per pixel by the exposure time per pixel
+===== Differential Count Rate =====
+see [[glossary:Differential Count Rate]]