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howto:2ffcs [2013/12/03 11:42] – Approved adminhowto:2ffcs [2015/11/04 09:41] (current) – admin
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-====== 2FFCS ======+{{tag> 2fFCS FCS}} 
 +~~TOC~~ 
 +====== 2fFCS ======
  
  
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 Correlation analysis that is applied to the fluctuations of the fluorescence intensity. The cross-correlation between the occurrence of events in two different detection volumes is used to introduce an absolute diffusion length into the analysis.  Correlation analysis that is applied to the fluctuations of the fluorescence intensity. The cross-correlation between the occurrence of events in two different detection volumes is used to introduce an absolute diffusion length into the analysis. 
 +
 +see [[glossary:FCS]] for a introduction on FCS.
  
 ===== Experimental Setup ===== ===== Experimental Setup =====
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 Occasionally, pinhole size can be used to adjust amount of photons to change the signal intensity and increase SNR. In addition to the "optimal" 1 AU, Pinhole 1-3 AU is the range of choice. Bigger pinhole give you stronger signal but with the compromised confocal effects. Occasionally, pinhole size can be used to adjust amount of photons to change the signal intensity and increase SNR. In addition to the "optimal" 1 AU, Pinhole 1-3 AU is the range of choice. Bigger pinhole give you stronger signal but with the compromised confocal effects.
  
-PQ usually uses a 50 micron pinhole (1.4 AU) to get maximum detection efficiency, somewhat sacrificing background rejection. +PicoQuant usually uses a 50 micron pinhole (1.4 AU) to get maximum detection efficiency, somewhat sacrificing background rejection. 
  
 === Two Foci === === Two Foci ===
howto/2ffcs.txt · Last modified: 2015/11/04 09:41 by admin