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howto:2ffcs [2013/11/27 10:02] – adminhowto:2ffcs [2014/04/09 20:02] – admin
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-====== 2FFCS ======+{{tag> 2fFCS FCS}} 
 +~~TOC~~ 
 +====== 2fFCS ====== 
  
 2fFCS is an abbreviation **2 F**ocus **F**luorescence **C**orrelation **S**pectroscopy. Dual focus FCS might also be used. 2fFCS is an abbreviation **2 F**ocus **F**luorescence **C**orrelation **S**pectroscopy. Dual focus FCS might also be used.
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 Occasionally, pinhole size can be used to adjust amount of photons to change the signal intensity and increase SNR. In addition to the "optimal" 1 AU, Pinhole 1-3 AU is the range of choice. Bigger pinhole give you stronger signal but with the compromised confocal effects. Occasionally, pinhole size can be used to adjust amount of photons to change the signal intensity and increase SNR. In addition to the "optimal" 1 AU, Pinhole 1-3 AU is the range of choice. Bigger pinhole give you stronger signal but with the compromised confocal effects.
  
-PQ usually uses a 50 micron pinhole (1.4 AU) to get maximum detection efficiency, somewhat sacrificing background rejection. +PicoQuant usually uses a 50 micron pinhole (1.4 AU) to get maximum detection efficiency, somewhat sacrificing background rejection. 
  
 === Two Foci === === Two Foci ===
howto/2ffcs.txt · Last modified: 2015/11/04 09:41 by admin