Updating from Windows 10 to Windows 11 should work smoothly, and all software should stay as it is. However it is a good idea to back-up the files mentioned below.
Please note: The Framegrabber MOU camera is not compatible with Windows 11 Windows 11 works with the newer USB MOU camera.
Updates for SymPhoTime 64 are free. You can always get the latest version from https://www.picoquant.com/products/category/fluorescence-microscopes/symphotime-64-fluorescence-lifetime-imaging-and-correlation-software
Updates of the SymPhoTime 64 should work smoothly by just installing from the installation EXE.