adapted.\\
**Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... hen applied to the lifetime graph(s) in the TCSPC histogram.\\
**Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a
.
- **Upper right: Fluorescence FRET Efficiency Histogram.** As the FRET efficiency image has not been calc... the right of the plot.
- **Lower left: Binding histogram.** As the FRET efficiency image has not been calculated yet, this histogram is still empty. This diagram can only be calculat... al decay kinetics.
- **Lower center/left: TCSPC histogram of all photons in the image.** In green (= datase
orial shows step-by-step, how to calculate a FRET histogram from single [[glossary:fret|FRET]]pairs detected ... s still empty.\\
- **Right:** FRET distribution histogram and the FRET radius histogram. As these distributions have not been calculated yet, this area is still empty... s region is used to calculate the FRET efficiency histogram. The second area shows the fluorescence response
ale can be adapted.\\
**3: Fluorescence Lifetime Histogram**\\
Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\
**4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\
**5: [[glossary:TCSPC]] Decay window**\\
By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
ale can be adapted.\\
**3: Fluorescence Lifetime Histogram**\\
Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\
**4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\
**5: [[glossary:TCSPC]] Decay window**\\
By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
ted.\\
**Upper right:** the Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... the lifetime graph(s) on the lower center (TCSPC histogram).\\
**Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a
ale can be adapted.\\
**3: Fluorescence Lifetime Histogram**\\
Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\
**4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\
**5: [[glossary:TCSPC]] Decay window**\\
By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
ted.\\
- **Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... the lifetime graph(s) on the lower center (TCSPC histogram).\\
- **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a
ale can be adapted.\\
**3: Fluorescence Lifetime Histogram**\\
Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\
**4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\
**5: [[glossary:TCSPC]] Decay window**\\
By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
ted.\\
- **Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... o the lifetime graph(s) in the [[glossary:TCSPC]] histogram.\\
- **Lower center/left:** [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red a
\\
- **Lower center:** Here the FRET efficiency histogram over the selected pixels will be plotted when the... ed.\\
- **Lower right:** Here the FRET distance histogram in terms of R0 will be plotted when the calculati... o 100% FRET efficiency.
* The FRET efficiency histogram and the Distances histogram are shown.
{{ calculate_ratiometric_fret-images_with_the_fret-image-script_Image
y-step, how to calculate a [[glossary:fret|FRET]] histogram from single FRET pairs detected under single mole... .\\
- **Lower center/right:** FRET distribution histogram and FRET radius histogram. As these distributions have not been calculated yet, this area is still empty.
... s region is used to calculate the FRET efficiency histogram. The unmarked white area shows the fluorescence r
e above highlighted in green. The photon counting histogram on the right displays the frequencies of the diff... ons, a lifetime fit can be performed of the TCPSC histogram of the measurement. The FLCS filters can be calcu... different parameters.
- **Upper center:** TCSPC histogram of the imported patterns. As we haven't loaded an... mage_11.png?384 }}
**Response:**
* The TCSPC histogram for this file appears in the "Patterns" graph in
olecules to calculate the static anisotropy and a histogram with the anisotropy distribution.
{{ youtube>Tyc... w opens which contains the anisotropy image and a histogram with the anisotropy distribution over the differe... tween -0.4 and 1.
- **Lower graph:** Anisotropy histogram over the different pixels.
**Note:**
The anisot... the display settings for the anisotropy frequency histogram in the corresponding controls.
{{ using_the_aniso
er/right:** TCPSC fitting graph showing the TCSPC histogram of the measured sample.
* In the decay form o... PC histograms of both files are loaded. The TCSPC histogram from the file "ATTO655_diff_FLCS-pattern.ptu is m... measurement of the dye Cy5 in water. In the TCSPC histogram it can be clearly seen that the lifetime of this