youtube>zHfGae_Pd8A?large }}
===== Background information =====
FLIM-FRET is a robust method to determi... ial_donors_Image_9.png }}
**Note:**\\
For more information about the different ROI selection tools, check
please check if they use current versions of file formats and/or programming libraries before you invest i... rpose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in model func... //github.com/PicoQuant/PicoQuant-Time-Tagged-File-Format-Demos| Demo Code for reading PicoQuants PTU File
====== Formatting Syntax ======
[[doku>DokuWiki]] supports some simple markup language, which tries to ma... ku>toolbar|quickbuttons]], too.
===== Basic Text Formatting =====
DokuWiki supports **bold**, //italic//... wiki:dokuwiki-128.png}}]]
Please note: The image formatting is the only formatting syntax accepted in link names.
The whole [[#images_and_other_files|image]] an
relation function =====
In general all relevant information is conveyed by the normalised autocorrelation ... or because of the internal flexibility of the conformation of the biomolecule the quencher comes closer t... luorescence one can infer the timescale of the conformational changes of the molecule under study. \\
{{:general:conformational_change_quencher.png?200|}}
==== A bit more
rts the image as an ASCII array using a text-file format. Thereby one array encodes the intensity values, ... mage series as a series of binary files. The data format of these files is described on the SymPhoTime Online help. The binary files contain the photon information of each TCSPC-channel and can be imported e.g.... icroTime system, or you transfer the image size information automatically via a handshake software, as in
Start menu, store the histograms in ''.phu'' data format. Older software versions of various timing electronics may use other data file formats. Possible file name extensions are ''.thd, .nhd,
ques and instrumentation. \\
* Many useful information can be found in [[https://www.microscopyu.com/... * [[ https://www.microscopyu.com/tutorials/imageformation-airyna |Numerical Aperture and Image Resolutio
youtube>Ka51hZNw3u8?large }}
===== Background information =====
FLIM-FRET is a robust method to determi... OCA-1, proteins involved in filopodia and vesicle formation ((Sample courtesy of S. Ahmed, T. Sudhaharan,
pen/process^
|''.ptu''|Raw data in a unified TTTR-format|<WRAP>
- Generated by a measurement
- Import ... ment file, contains manually entered text (e.g. information about the experiment)|Menu: **File → Create Co... ta) file opens a file viewer, in which you find information about the file type and acquisition settings
<imgcaption image1| ''.ptu'' file information and comment >
{{info_image.png?800| }}
</imgca
youtube>0Ht_L3a53E0?large }}
===== Background Information =====
Fluorescence Cross-Correlation Spectros... o vary in their fluorescence lifetime, for more information please check the tutorials for Fluorescence Li