====== Fluorescence Correlation Spectroscopy (FCS) ======
Fluorescence correlation spectroscopy (FCS) is a correlation analysis of fluctuation of the fluorescence intensity. The analysis provides parameters of th... (molecules) in solution. In this application, the fluorescence emitted from a very tiny space in solution contai
ime for TCSPC devices used for the measurement of fluorescence decays are in the range of 25 to 90 ns.
Let's t... an example a laser repetition rate of 40 MHz. The fluorescence molecules receive every 25 ns a laser pulse. Cons... stics: The probability of detecting more than one fluorescence photon per excitation pulse is negligible.
* [
F**luorescence **LI**fetime **M**icroscopy. ([[wp>Fluorescence-lifetime imaging microscopy]]).
In FLIM addition... lse color plot is used to visualize the average [[fluorescence lifetime]] in each pixel of the image.
===== FLI