cted with the donor construct by performing a ROI-fit with the FLIM-analysis. The amplitude weighted av... y lifetime and also to perform the pixel-by-pixel fit to calculate the FRET efficiency.
===== Step-by-... *Note:**\\
The software offers the possibility to fit the data using a n-exponential tailfit, a n-exponential reconvolution fit or a rapid reconvolution fit. A tailfit can be us
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This tutorial shows step-by-step, how to fit a selected model to a decay curve in order to get... As an example, a single exponential reconvolution fit is used to determine the lifetime of ATTO655 dilu... al tailfit or a n-exponential reconvolution model fit. A tailfit can be used when the expected lifetime... strument response function. Still a reconvolution fit is usually preferable, because the complete decay
can be used to perform a pixel-by-pixel lifetime fit of a FLIM image and how to extract and read the r... ited fitting model is chosen and a pixel-by-pixel fit is performed.
===== Step-by-step Tutorial ====... lly want to have in a pixel in order to perform a fit. If there are too few photons in a pixel, a decent fit result cannot be expected. It is also possible to