cted with the donor construct by performing a ROI-fit with the FLIM-analysis. The amplitude weighted av... y lifetime and also to perform the pixel-by-pixel fit to calculate the FRET efficiency.
===== Step-by-... the right of the plot.\\
**Lower left:** Lifetime fitting parameters. Here lifetime fitting models can be defined. Fitting is then applied to the lifetime g
elation Software accepting pt3 and ptu files. for fitting see https://dwaithe.github.io/FCSfitJS/
[[https://dwaithe.github.io/FCSfitJS/| FCS fit JS]]
* [[software:FLIMFit]]. FLIMfit is an ope... hical user interface for analyzing, plotting, and fitting of time tagged data.[[https://eta.readthedocs... ub.com/skyjur/picoharp300-curvefit-ui|TCSPC curve fitting for phd files (Python)]]
* [[ https://g
mPhoTime}}
~~NOTOC~~
====== SymPhoTime Lifetime Fitting ======
===== Summary =====
This tutorial shows step-by-step, how to fit a selected model to a decay curve in order to get... As an example, a single exponential reconvolution fit is used to determine the lifetime of ATTO655 dilu... on the left side of the main window.
{{ lifetime_fitting_using_the_tcpsc_fitting_script_Image_1.png }}
mPhoTime MicroTime LSM_upgrade}}
====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTi... can be used to perform a pixel-by-pixel lifetime fit of a FLIM image and how to extract and read the r... //figshare.com/s/4957fcfa684daef86c23]], a suited fitting model is chosen and a pixel-by-pixel fit is performed.
===== Step-by-step Tutorial =====
==== S