cted with the donor construct by performing a ROI-fit with the FLIM-analysis. The amplitude weighted av... y lifetime and also to perform the pixel-by-pixel fit to calculate the FRET efficiency.
===== Step-by-... itting model.
{{ :howto:flim-fret-multiexpd_reconvfit.png?400 |}}
**Response:**\\
* In the TCSPC w... ameter table.
{{ :howto:flim-fret-multiexpd_reconvfit_response.png |}}
**Note:**\\
The software offers
hub.com/PicoQuant/EasyTau-Scripts
[[software:FluoFit]] \\
Decay curve analysis software
===== Open Sou... e-Molecule Spectroscopy]]
* [[software:PyCorrFit]] is a general-purpose FCS evaluation software t... ablished Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in model functions, cove... g (DLS).
* [[http://www.dkfz.de/Macromol/quickfit|QuickFit 3.0]]: QuickFit 3 is our open-source dat
=====
This tutorial shows step-by-step, how to fit a selected model to a decay curve in order to get... As an example, a single exponential reconvolution fit is used to determine the lifetime of ATTO655 dilu... offers the possibility to use a n-exponential tailfit or a n-exponential reconvolution model fit. A tailfit can be used when the expected lifetimes are sign
can be used to perform a pixel-by-pixel lifetime fit of a FLIM image and how to extract and read the r... ited fitting model is chosen and a pixel-by-pixel fit is performed.
===== Step-by-step Tutorial ====... lly want to have in a pixel in order to perform a fit. If there are too few photons in a pixel, a decent fit result cannot be expected. It is also possible to