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create_time_gated_image [2020/10/14 11:45] – created buschmanncreate_time_gated_image [2020/10/14 12:10] – [Step-by-Step Tutorial] buschmann
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   * The image is updated. It still shows the rainbow scale. Features, which had a short lifetime in the ungated image are enhanced. If you wish, you can adjust the rainbow scale.   * The image is updated. It still shows the rainbow scale. Features, which had a short lifetime in the ungated image are enhanced. If you wish, you can adjust the rainbow scale.
  
 +{{:flim-image_after_gate_selection_3.png|}}
  
 + * To get a pure time-gated intensity image, set to greyscale and select "Events" as parameter to show.
 +
 +{{:flim-image_after_time-gate_selection4.png|}}
 +
 + * The result image can be saved in SymPhoTime by clicking "Save Result" or be exported via right-mouse click, while the mouse point is placed over the image.
 +
 +{{:gatedimageexport.png|}}
 +
 + * If a second time-gated image should be selected, either just select a new time gate as described above and repeat these steps. 
 + * If you want to simply create a second time-gated image with the complementary photon selection, just click on the white area in the gate-selection graph and click "fast-FLIM" again. Then you can select the grey-scale instead of the rainbow scale to display the "Events" and get a image created from the later photons. 
 +
 +{{:gatedimage_latephotons.png|}}
  
  
create_time_gated_image.txt · Last modified: 2020/10/14 12:10 by buschmann