/pixel is quite low in this image, select a Pixel Binning of 4 points.
* Press "Calculate FastFLIM" to up... lcfastflim.png?400 |}}
**Response:**\\
The pixel binning is applied to the image and the image is recalcul
ROI ====
* In parameter panel, enter a frame binning of 10 in order to decrease processing time and in... e bigger cells are located.
{{ howto:roi0_10framebinning_2.png |}}
* Tick the "Select in all Frames" c... TCSPC decay window.
{{ howto:rois_and_decays_afterbinning_2.png |}}
==== Perform an Initial Lifetime Fit o... residuals and χ².\\
{{ howto:monoexpo_roi0_10framebinning_2.png |}}
**Note:**\\
Usually, a decent fit is
sts of 102 individual frames. By default, a frame binning of 102 is selected. Thus, the measurement is plot... * Shift the parameter pannel up and enter a frame binning of 6 and a (pixel) binning of 3 to decrease processing time and increase photon numbers per pixel.
{{ visu... ge stack is calculated according to the set pixel binning, in which always 6 frames are combined. As the co
f the image pixels large enough or should a pixel binning be perfomed? In that case increase the Binning from "1 Points" to a higher value and click "Calculate".
{{
* In the "Trace Settings" on the left, change the binning to 0,5 ms.
**Note:** If you are unsure which binning to select, calculate the FCS curve and check the dif
* In the "Trace Settings" on the left, change the binning to 0.5 ms.
**Note:** If you are unsure which binning to select, calculate the FCS curve and check the dif
a 3-exponential model. Therefore, perform a pixel binning by increasing the "Binning" to two points and click "Calculate FastFLIM".
{{ lifetime-fitting_using_the_fl
following parameters can be adapted:
* Pixel binning by changing the "Binning", e.g. to twopoints.
* Select the second excitation peak by clicking on the w
ted yet, this area is still empty.
* Select a "Binning" of 10 ms.
* Click "Calculate".
{{ using_the_i... ime trace window opens, click "Calculate" using a binning of 1 ms as set by default.
{{ using_the_intensity