me 64 can be used to calculate pixel-by-pixel the average [[glossary:fret|FRET]] efficiency in an image con... ed with Cerulean/YFP-constructs. To calculate the average FRET-efficiency, first the donor only lifetime is... it with the FLIM-analysis. The amplitude weighted average donor lifetime is used to calculate the average FRET-efficiency using the "Lifetime FRET Image" analysis.
ncodes the intensity, while the color encodes the average "Fast FLIM" lifetime, i.e. the mean arrival times... the dark background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the c... change, as now the calculated intensity weighted average lifetime "tau_Av_Int" is displayed, in contrast t
encodes the intensity while the color encodes the average "Fast FLIM" lifetime, i.e. the mean arrival times... to display (e.g. T_av_Int, the intensity-weighted average lifetime, which - neglecting background photons –
ncodes the intensity, while the color encodes the average "Fast FLIM" lifetime, i.e. the mean arrival times... the dark background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the c
cenarios are illustrated. In <imgref figure1>A on average only 1 fluorescent molecule resides in the detect... y when the molecule is in) in comparison with the average value. In <imgref figure1>B a few molecules reside in the detection volume on average, and thus the relative fluctuations will be small... re a few molecules in focus) in comparison to the average. This difference in the relative fluctuations is
ncodes the intensity, while the color encodes the average "Fast FLIM" lifetime, i.e. the mean arrival times... the dark background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the c... change of the fit values and compare for instance average lifetime of your ROIs.
* Select the intensity ... lot_10framebinning_2.png |}}
**Response:**\\
The average lifetime, you can see that after adding the sugar
ncodes the intensity, while the color encodes the average "Fast FLIM" lifetime, i.e. the mean arrival times... the dark background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the c
encodes the intensity while the color encodes the average "Fast FLIM" lifetime, i.e. the mean arrival time ... tor, the binding ratio has a direct effect on the average lifetime.
{{ :howto:flim-fret-1expd_calcfret_resp
* G(0)
* Num. Molecules (calculated from the average countrate and G(0))
* mol. Brightness (in kcps/... ideal system should equal the intensity weighted average lifetime [τ av. int.]. Usually, fitted lifetimes