me 64 can be used to calculate pixel-by-pixel the average [[glossary:fret|FRET]] efficiency in an image con... ed with Cerulean/YFP-constructs. To calculate the average FRET-efficiency, first the donor only lifetime is... it with the FLIM-analysis. The amplitude weighted average donor lifetime is used to calculate the average FRET-efficiency using the "Lifetime FRET Image" analysis.
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===== Process Several Files and Calculate Averages =====
* If several raw data files are marked ... tion of the fitting values. It also calculates an average for each fitting value over the data sets. In our
ncodes the intensity, while the color encodes the average "Fast FLIM" lifetime, i.e. the mean arrival times... the dark background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the c... change, as now the calculated intensity weighted average lifetime "tau_Av_Int" is displayed, in contrast t