for_multi-exponential_donors_Image_2.png? }}
* Select the "Analysis" tab and in there, open the drop do... and open the context menu by a right mouse click. Select the "Free ROI" tool.
{{ flim-fret-calculation_for... 0 }}
* In the decay form on the lower left, select n-exponential reconvolution as fitting model.
{{ ... of the different parameters.
* Under "Decay" select "ROI 0".
**Response:**\\
The TCSPC curve of ROI 0
formed.
===== Step-by-step Tutorial =====
==== Select a file and start the analysis ====
* Start SymP... ey_Cells_FLIM.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the *... st by adapting "Min" and "Max" values.
==== Select ROIs ====
* Open the "Region of Interest" drop... n you are content with the selection, press OK to select these pixels of the image as Region of Interest (
VV8?large }}
===== Step-by-Step Tutorial =====
Select a file and start the script
* Start [[products... sing_the_tcpsc_fitting_script_Image_2.png }}
* Select the "Analysis" tab and in there, open the drop do... ured sample.
* In the decay form on the left, select n-exponential reconvolution as fitting model.
**R... a right mouse click to open the context menu and select "Copy" or simple use "<CRTL>" + "C" to copy the f