ile|<WRAP>
- Generated from the online analysis selected during the measurement (the Countrate-Display i... the values calculated during an online analysis, select the "**Comment**"-tab.
The kind and amount of t...
* Max. Photons (in the brightest pixel for the selected data channel)
* Max. Countrate (calculated ta... ween the different display options can be done by selecting the desired display option above the scale.
^
rmed.\\
===== Step-by-step Tutorial =====
==== Select a file and start the analysis ====
* Start Sym... ey_Cells_FLIM.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the *... meters**\\
Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(... st by adapting "Min" and "Max" values.
==== Select a file and start the analysis ====
* In the FL
vLQW4nY?large }}
===== Step-by-Step =====
==== Select a file and start the script ====
* Start [[pro... fitting_using_the_flim_script_Image_2.png }}
* Select the "Analysis"-tab and in there, open the drop do... ime contrasts in the pollen become visible.
==== Select 3 Regions of Interest (ROI) ====
* Keep the m... ing_using_the_flim_script_Image_7.png?349 }}
* Select a ROI tool in the context menu, e.g. the "FreeROI
6c23]].
===== Step-by-Step Tutorial =====
==== Select a file and start the analysis ====
* Start Sym... 'MicroBeads.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the **"... meters**\\
Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(... t by adapting "Min" and "Max" values.
==== Select ROIs ====
* On top left, under Region of Int
nd an overall decay from the raw data file.
==== Select Patterns ====
**Note:** Once an image is generated, the next step is to select the patterns which are to be used for decomposing... g_Image_7.png?600 }}
* In the right-click menu select the magic wand ROI (region-of-interest).
{{ pattern_matching_Image_8.png?600 }}
* Select the innermost contour line by clicking on it.
{{
for_multi-exponential_donors_Image_2.png? }}
* Select the "Analysis" tab and in there, open the drop do... and open the context menu by a right mouse click. Select the "Free ROI" tool.
{{ flim-fret-calculation_for... **\\
For more information about the different ROI selection tools, check the tutorial [[howto:roi_fitting_... he left mouse button activated while circling the selected part of the image. Then release the left mouse
f86c23]].
===== Step-by-Step Tutorial =====
==== Select a file and start the analysis ====
* Start Sym... ck_MDCK_Cells.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the *... meters**\\
Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(... ram.\\
==== Define image stack options and select ROI ====
* In parameter panel, enter a frame
, which can be amplified
{{:flim-image_time-gate_selection.png|}}
* Now a time gat can be defined by m... a time can be entered in the field above. Here we select the photons, which arrive roughly with the laser pulse. The green region in the image is the selected region.
{{:flim-image_time-gate_selection2.png|}}
* Click "OK" to close the window. Now the selecti
formed.
===== Step-by-step Tutorial =====
==== Select a file and start the analysis ====
* Start SymP... ey_Cells_FLIM.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the *... meters**\\
Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(... st by adapting "Min" and "Max" values.
==== Select ROIs ====
* Open the "Region of Interest" drop
This tutorial shows step-by-step, how to fit a selected model to a decay curve in order to get the life... VV8?large }}
===== Step-by-Step Tutorial =====
Select a file and start the script
* Start [[products... sing_the_tcpsc_fitting_script_Image_2.png }}
* Select the "Analysis" tab and in there, open the drop do... ured sample.
* In the decay form on the left, select n-exponential reconvolution as fitting model.
**R
d laser.
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[pro... separation_of_2_species_with_Image_2.png }}
* Select the "Analysis" tab and in there, open the drop do... as evenly splitted onto two detectors. Therefore, select both detectors in the definition of "Channel A":
... ration_of_2_species_with_Image_10.png?383 }}
* Select the file ''Cy5_diff_IRF+FLCS-pattern.ptu'' and cl
on.
\\
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[pro... g_the_anisotropy_image_script_Image_2.png }}
* Select the "Analysis" tab and in there, open the drop do... measuring a randomly aligned ensemble due to photoselection the anisotropy varies with in the limits of -... ivate the context menu by a right mouse click and select "Show data reader". The intensity of the pixel wh
}}
\\
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[pro... e_intensity_time_trace_script_Image_2.png }}
* Select the "Analysis" tab and in there, open the drop do... en calculated yet, this area is still empty.
* Select a "Binning" of 10 ms.
* Click "Calculate".
{{ ... Image_6.png }}
**Response:** As activated under "Select data channels", two intensity plots are calculate
large }}
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[pro... lick.
{{ visualizing_dynamics_Image_2.png }}
* Select the "Analysis" tab and in there, open the drop do... cess indicated by blurring of certain GUVs.
==== Select the Processing Options and create and export an i... ual frames. By default, a frame binning of 102 is selected. Thus, the measurement is plotted as one image