vLQW4nY?large }}
===== Step-by-Step =====
==== Select a file and start the script ====
* Start [[pro... fitting_using_the_flim_script_Image_2.png }}
* Select the "Analysis"-tab and in there, open the drop do... ime contrasts in the pollen become visible.
==== Select 3 Regions of Interest (ROI) ====
* Keep the m... ing_using_the_flim_script_Image_7.png?349 }}
* Select a ROI tool in the context menu, e.g. the "FreeROI
6c23]].
===== Step-by-Step Tutorial =====
==== Select a file and start the analysis ====
* Start Sym... 'MicroBeads.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the **"... t by adapting "Min" and "Max" values.
==== Select ROIs ====
* On top left, under Region of Int... d Fast Lifetime color code. This will help you to select your ROI or threshold easier.
{{ howto:adaptcont
for_multi-exponential_donors_Image_2.png? }}
* Select the "Analysis" tab and in there, open the drop do... and open the context menu by a right mouse click. Select the "Free ROI" tool.
{{ flim-fret-calculation_for... 0 }}
* In the decay form on the lower left, select n-exponential reconvolution as fitting model.
{{ ... of the different parameters.
* Under "Decay" select "ROI 0".
**Response:**\\
The TCSPC curve of ROI 0
rmed.\\
===== Step-by-step Tutorial =====
==== Select a file and start the analysis ====
* Start Sym... ey_Cells_FLIM.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the *... st by adapting "Min" and "Max" values.
==== Select a file and start the analysis ====
* In the FL... nu.\\
- If you want to start again from scratch select "Select all as ROI".\\
* In this step, as an e
f86c23]].
===== Step-by-Step Tutorial =====
==== Select a file and start the analysis ====
* Start Sym... ck_MDCK_Cells.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the *... ram.\\
==== Define image stack options and select ROI ====
* In parameter panel, enter a frame ... k on the FastFLIM image and from the context menu select "FreeROI" tool.\\
* In order to quantify tensi
formed.
===== Step-by-step Tutorial =====
==== Select a file and start the analysis ====
* Start SymP... ey_Cells_FLIM.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the *... st by adapting "Min" and "Max" values.
==== Select ROIs ====
* Open the "Region of Interest" drop... n you are content with the selection, press OK to select these pixels of the image as Region of Interest (
the values calculated during an online analysis, select the "**Comment**"-tab.
The kind and amount of t... place the cursor above the scale, right click and select **"Scale (ALL)"**. {{ scaling-menu-image.png?200|... place the cursor above the scale, right click and select **"Clip Outliners"**. This feature ignores outlin... ion.
* If you want to start from scretch again, select **“Select all as ROI”**
* you can also invert
nd an overall decay from the raw data file.
==== Select Patterns ====
**Note:** Once an image is generated, the next step is to select the patterns which are to be used for decomposing... g_Image_7.png?600 }}
* In the right-click menu select the magic wand ROI (region-of-interest).
{{ pattern_matching_Image_8.png?600 }}
* Select the innermost contour line by clicking on it.
{{
d laser.
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[pro... separation_of_2_species_with_Image_2.png }}
* Select the "Analysis" tab and in there, open the drop do... as evenly splitted onto two detectors. Therefore, select both detectors in the definition of "Channel A":
... ration_of_2_species_with_Image_10.png?383 }}
* Select the file ''Cy5_diff_IRF+FLCS-pattern.ptu'' and cl
\\
\\
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[pro... ingle_pair_fret_distributions_Image_2.png }}
* Select the "Analysis" tab and in there, open the drop do... ms.
**Note:** If you are unsure which binning to select, calculate the FCS curve and check the diffusion ... ivate the context menu by a right mouse click and select "Show Data Reader/Select Curve".
{{ calculate_ra
rs.
\\
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[pr... ons_using_the_pie-fret_script_Image_2.png }}
* Select the "Analysis" tab and in there, open the dropdow... ms.
**Note:** If you are unsure which binning to select, calculate the FCS curve and check the diffusion ... ivate the context menu by a right mouse click and select "Show Data Reader/Select Curve".
{{ calculate_ra
}}
\\
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[pro... e_intensity_time_trace_script_Image_2.png }}
* Select the "Analysis" tab and in there, open the drop do... en calculated yet, this area is still empty.
* Select a "Binning" of 10 ms.
* Click "Calculate".
{{ ... Image_6.png }}
**Response:** As activated under "Select data channels", two intensity plots are calculate
on.
\\
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[pro... g_the_anisotropy_image_script_Image_2.png }}
* Select the "Analysis" tab and in there, open the drop do... ivate the context menu by a right mouse click and select "Show data reader". The intensity of the pixel wh... tivate the context menu with a right mouse click, select a ROI selection tool and mark the regions you wan
VV8?large }}
===== Step-by-Step Tutorial =====
Select a file and start the script
* Start [[products... sing_the_tcpsc_fitting_script_Image_2.png }}
* Select the "Analysis" tab and in there, open the drop do... ured sample.
* In the decay form on the left, select n-exponential reconvolution as fitting model.
**R... a right mouse click to open the context menu and select "Copy" or simple use "<CRTL>" + "C" to copy the f