{{tag>howto IRF acquisition video Nikon LSM_upgrade}}
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====== How to Measure the Instrument Response Function (IRF) ======
(For an explanation of the term Instrument Response Function see [[glossary:IRF]])
The following video shows how to measure na IRF on confocal microscopes.
{{youtube>cjSgCBe3ReM?la
estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the TCSPC histogram.
* As dat... }
**Response:**\\
* In the TCSPC window, the IRF is displayed in bright red, the data fitting limi... C window.
* The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the f
estimation of the instrument response function (=IRF) is shown. The IRF reconstruction is deducted from the rising edge of the [[glossary:TCSPC]] histogram... 00 |}}
** Response:**\\
In the TCSPC window, the IRF is displayed in bright red, the data fitting limi... TCSPC window.\\
The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the f
del.
**Response:**
* In the TCSPC window, the IRF is displayed in bright red and the data fitting l... C window.
* The new fitting parameters "Shift IRF" and "Bkgr IRF" (=background IRF) appear in the fitting parameter table.
{{ lifetime_fitting_using_the_tcpsc_fitting_