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Fulltext results: flim_fret_calculation_for_multi_exponential_donors 33 Hits , Last modified: 6 months ago {{tag>tutorial howto analysis FLIM imaging FRET SymPhoTime}}
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====== FLIM-FRET Calculation fo... _for_FRET.ptu''. Thereby, a new Window opens:
{{ :howto :flim-fret-multiexpd_analysis_flim.png |}}
**Note... channel the donor emission has been recorded.
{{ :howto :flim-fret-multiexpd_ch2.png?400 |}}
* Press "C... transfected only with the donor constructs.
{{ :howto :flim-fret-multiexpd_enahncedcontrast.png?400 |}}
lifetime-fitting_using_the_flim_script 15 Hits , Last modified: 10 months ago {{tag>howto tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}}
====== Lifetime-Fitting Using the ... e FLIM analysis. Thereby, a new Window opens:
{{ howto :kidneyfullscreen3.png |}}
**Note:**\\
The window... I" checkbox and click "ROI from threshold".\\
{{ howto :activateroi.png |}}
**Response:**\\
A large win... a threshold for an image analysis visually.
{{ howto :selectthreshold_kidney3.png |}}
**Note:**\\
Lef antibunching_measurements 3 Hits , Last modified: 9 months ago {{tag>howto antibunching correlation}}
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====== How to Perform Antibunching Measurements ======
... = Software Settings during Acquisition ===
[{{ howto :100210_06_antibunching.png?400|Figure 1: Correlat... ters]]. Laser rep. rate 10 MHz for details}}]
[{{ howto :100210_step_05_antibunching.png?400|Figure 2: Cor how_to_measure_the_instrument_response_function_irf 1 Hits , Last modified: 9 months ago {{tag>howto IRF acquisition video Nikon LSM_upgrade}}
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====== How to Measure the Instrument Resp lifetime_fitting_using_the_tcpsc_fitting_script 1 Hits , Last modified: 9 months ago {{tag>howto analysis lifetime tutorial SymPhoTime}}
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====== SymPhoTime Lifetime Fitting =====