Phasor Analysis

Phasor Analysis is currently not included in SymPhoTime64. However, you can use Globals developed by the Laboratory for Fluorescence Dynamics to analyse FLIM data via the Phasor approach.

• Download Globals for Images
• Install SimFCS according to the documentation: http://www.lfd.uci.edu/globals/

• Start FLIM analysis
• Calculate a fastFLIM image
• Select Export Binary ¹⁾

• follow the Reading PicoQuant BIN files tutorial of the Globals Software package to import the BIN files.
• follow the Phasor Analysis Tutorial of the Globals Software.

• Deriving the laser frequency from PicoQuant BIN files
• Function: Read the PicoQuant file
  
  

Users of SymPhoTime 32 with a PicoHarp 300 ,please refer to:

• Using PicoQuant PT3 format for FLIM
• Reading PicoQuant PT3 files for FCS and lifetime FCS

Additional Tutorials for the Globals software can be found here: http://www.lfd.uci.edu/globals/tutorials/