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This tutorial assumes SymPhoTime V2.0
===== General UI Philosophy =====
* right clicking on graphs... ent >
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The general file information contains for example:
* the n... specially important for LSM upgrade kits where in general several frames are recorded, while for an image a... on the scaling of the color-encoded component (in general, the lifetime). Changing the color scale can also
r to the fluorescence emission wavelength.
==== General recipe ====
* Create a saturated aqueous KI (p... ay.
Our guess is that this quenching trick works generally. Specifically, we found that classic Fluorescei... the color dependence of SPAD detectors, which are generally used in microscopy. Furthermore, even with dete
principles are similar as for any FLIM-recording.
General recording of FLIM images can also be found using the link below.
The general principles of the data acquisition are analogous