e [[http://www.picoquant.com/images/uploads/page/
files/7267/appnote_flim_fret.pdf]].
The example image ... 4 software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:**\\
... going through this tutorial.
**Response:**\\
The files of the sample workspace are displayed in the wor... onential_donors_Image_1.png }}
* Highlight the file ''CENP-labelled _cells_for_FRET.ptu'' by a single
}}
===== Step-by-Step Tutorial =====
Select a file and start the script
* Start [[products:SymPho... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... e going through this tutorial.
**Response:** The files of the sample workspace are displayed in the wor... _fitting_script_Image_1.png }}
* Highlight the file ''ATTO655_diff_FLCS-pattern.ptu'' by a single mou
===== Step-by-step Tutorial =====
==== Select a file and start the analysis ====
* Start SymPhoTime ... rsions).
* Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.\\
**Response:**\\
The files of the sample workspace are displayed in the wor... e left side of the main window.
* Highlight the file ''Kidney_Cells_FLIM.ptu'' by a single mouse cli
arge }}
===== Step-by-Step =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... e going through this tutorial.
**Response:** The files of the sample workspace are displayed in the wor... the_flim_script_Image_1.png }}
* Highlight the file ''DaisyPollen_cells_FLIM.ptu'' by a single mouse
===== Step-by-step Tutorial =====
==== Select a file and start the analysis ====
* Start SymPhoTime... rsions).
* Open the “Tutorials” workspace via **File -> Open Workspace** from the main menu.\\
**Response:**\\
The files of the sample workspace are displayed in the wor... e left side of the main window.
* Highlight the file ''Kidney_Cells_FLIM.ptu'' by a single mouse cli
ng used in the analysis. Therefore, highlight the file with the measured IRF (single mouse click on the .ptu-file)\\ An example measured IRF can be found in Tutori... |}}
6. Click on “Export Decay as IRF”. Rename the file accordingly. The IRF can now be used for the fitt... laser settings (Please see `System Specification File` which is left by installation in your lab. The m
===== Step-by-Step Tutorial =====
==== Select a file and start the analysis ====
* Start SymPhoTime... on 2.5).
* Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.\\
**Response:**\\
The files of the "Tutorials" workspace are displayed in th... e left side of the main window.
* Highlight the file ''Hyperosmotic_Shock_MDCK_Cells.ptu'' by a singl
===== Step-by-Step Tutorial =====
==== Select a file and start the analysis ====
* Start SymPhoTime... on 2.5).
* Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.
**Response:**\\
The files of this workspace are displayed in the workspace... left side of the main window.
* Highlight the file ''MicroBeads.ptu'' by a single mouse click.
p150]] is controlled via EasyTau 2. Such ''.etc'' files can be opened directly, using the {{:howto:ff.pn... of various timing electronics may use other data file formats. Possible file name extensions are ''.thd, .nhd, .phd'' and ''.hhd'' In order to analyze such files, they have to be imported into an already existi
==== Data file import ====
Create a new workspace or open an existing one:
{{ :howto:et2_createopen.png | File menu }}
Then select Import Files. A new dialog opens, where the data file is selected:
{{ :howto:et2_fileimport.png | Selects the file
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File -> Open Workspace" from the main menu.
**Note:**... e going through this tutorial.
**Response:** The files of the sample workspace are displayed in the wor... lizing_dynamics_Image_1.png }}
* Highlight the file ''GUVs.ptu'' by a single mouse click.
{{ visualiz
e: [[http://www.picoquant.com/images/uploads/page/files/7350/appnote_flim_overview.pdf|Fluorescence Life... oTime 64]].
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:**
T... going through this tutorial.
**Response:** The files of the sample workspace are displayed in the wor... -fret-1expd_samples_ws.png |}}
* Highlight the file ''FRET_GFP and mRFP.ptu'' by a single mouse click
In detail, this tutorial covers:
* Different file-types used or generated by SymPhoTime64
* Image... tional options and tools otherwise hidden
===== File-Type Overview =====
^Workspace: may contain these Filetypes ^ Content of the file ^ How to get such a file in the workspace ^ How to open/process^
|''.ptu''|R
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... e going through this tutorial.
**Response:** The files of the sample workspace are displayed in the wor... calculate_fccs_Image_1.png }}
* Highlight the file ''IBA488+547_crosslinked.ptu'' by a single mouse
mage|Structure of the pre-histogrammed Image Data File]]))
{{ :howto:bin_export_spt64.png?600 |}}
=====... 013.08.01%20-%20Reading%20the%20PicoQuant%20BIN%20file.pdf|Reading PicoQuant BIN files]] tutorial of the Globals Software package to import the BIN files.
* follow the [[https://download.lfd.uci.edu/t