ww.mpc.hhu.de/software/3-software-package-for-mfd-fcs-and-mfis|Software Package for Multiparameter Fluo...
* [[software:PyCorrFit]] is a general-purpose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-i... a wide range of applications in standard confocal FCS. In addition, it contains equations dealing with
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==== Fluorescence Correlation Spectroscopy (FCS) ====
[[general:fluorescence_correlation_spectro... py-_a_short_introduction| A short introduction to FCS with suggestions for further reading]]
[[howto:Performing an FCS Measurement with an Olympus FV1200 upgrade kit]]
[[howto:calculate_and_fit_fcs_traces_with_the_fcs_script|Calculate and fit FCS
======MT200 FCS Measurement ======
{{tag>howto MicroTime200 video tutorial}}
fluorescence correlation spectroscopy (FCS) is a quantitative measurement of the resolution
{{tag>MicroTime LSM Theory SymPhoTime FCS Correlation}}
~~TOC~~
====== Introduction to Fluorescence C... und =====
Fluorescence Correlation Spectroscopy (FCS) is founded on the analysis of the fluctuations o... rescence intensity detected from a given system. FCS was introduced in the 1970's by Magde, Elson and ... ules. In short time, the theoritical framework of FCS was expanded to incorporate also the study of rot
the script with the stored result
- For online FCS-results, the calculated molecular brightness can ... result-file opens with a preview display (Image, FCS-traces, TCSPC-curve or Time Trace - depending on ... me]], the image consists of a single frame)
=== FCS (for each of the max.2 data channels) ===
<WRAP>
<imgcaption CommentFCS| Comment Tab of an FCS online analysis result>{{onlinefcs-3.png?300|}}</
{{tag> analysis howto tutorial FCS FCCS Cross-Correlation SymPhoTime}}
~~NOTOC~~
====== Calculate FCCS Traces with the Grouped FCS Script ======
===== Summary =====
This tutoria... dye-labeled DNA-oligonucleotide using the Grouped FCS script.
{{ youtube>0Ht_L3a53E0?large }}
===== B... ngle mouse click.
**Note:** This file contains a FCS measurement of a FCCS positive control, a DNA oli
9%20-%20Reading%20PicoQuant%20PT3%20files%20for%20FCS%20and%20lifetime%20FCS.pdf| Reading PicoQuant PT3 files for FCS and lifetime FCS]]
Additional Tutorials for the Globals software can be found here: [[http://www.lfd
wto analysis calibration PSF confocal-volume VEFF FCS SymPhoTime}}
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====== Calibrate the Confocal Volume for and with FCS ======
===== Summary =====
This tutorial shows... by-step, how to calibrate the confocal volume for FCS measurements with a calibration dye. Therefore, a FCS curve is calculated with the FCS script, that is