etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte
torage:
<code>
keep at ~ 4°C, renew the solution after one month
</code>
Spectra:
<code>
excitation fr... ctrometers, the IRF is usually measured before or after each measurement.
In microscopy-applications, th
etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... he pixel decay in grey, the background before and after the decay is practically 0.Therefore, we can also
etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... a second array encodes the Fast Lifetime values. After removal of the header and the unwanted array, ind... ROI 2\\
* Keep the cursor over the graph, and after a right mouse click, select from the context menu
etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... ponse:**\\
The average lifetime, you can see that after adding the sugar, has been dropped gradually abou... more arrays depending on the fitting model used. After removal of the header and the unwanted array, ind
etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... here is still a bit of deviation in the residuals after the IRF in the decay window. This however can be ... oi1.png |}}
* If you compare the result image after the fit with the fast lifetime image above, you w
ll end up in dedicated ''.etc'' data containers.
After importing ''Decay_Coumarin_6.phu'', the workspace... ike this:
{{ :howto:et2_workspace1.png |}}
\\
\\
After expanding the data container:
{{ :howto:et2_works
etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... more arrays depending on the fitting model used. After removal of the header and the unwanted array, ind
e acquisition (you cannot change this information after the acquisition)
More detailed information can b... y inside the ''.ptu'' file and cannot be modified after the measurement (see <imgref image1>).
==== Re...
</WRAP>|
* some parameters are only available after performing a FLIM-Fit (i.e. the different lifetim... "FastLT" along the line is displayed. \\ {{:howto:after_selection_line_profile.jpg?350|}}
Note: The