etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte
torage:
<code>
keep at ~ 4°C, renew the solution after one month
</code>
Spectra:
<code>
excitation fr... ctrometers, the IRF is usually measured before or after each measurement.
In microscopy-applications, th
are however artifacts caused by photons emitted (after the detection of a genuine photon) by one of the ... nd SPAD. The distance of the expected dip and the afterflash peak is characteristic for the setup.
To get rid of the afterflash peak, it has been proven useful to introduce... ectly in front of each [[Glossary:SPAD]] E.g. the afterflash of MPD SPADs for Atto488 (485 nm excitation,
etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... he pixel decay in grey, the background before and after the decay is practically 0.Therefore, we can also
etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... a second array encodes the Fast Lifetime values. After removal of the header and the unwanted array, ind... ROI 2\\
* Keep the cursor over the graph, and after a right mouse click, select from the context menu
sity F at time t with the fluuorescence intensity after a lag time τ divided by the mean fluorescence int... nfocal volume can be determined ($\ref{eqVeff}$). After the calibration of the detection volume one can u... robability of having the same level of the signal after lag time, $t=t_o+\tau$. The main difference of FC
etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... the TCSPC decay window.
{{ howto:rois_and_decays_afterbinning_2.png |}}
==== Perform an Initial Lifetim... ponse:**\\
The average lifetime, you can see that after adding the sugar, has been dropped gradually abou... more arrays depending on the fitting model used. After removal of the header and the unwanted array, ind
etime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwise, inte... here is still a bit of deviation in the residuals after the IRF in the decay window. This however can be ... oi1.png |}}
* If you compare the result image after the fit with the fast lifetime image above, you w
enter tip 40%>
Do not forget to press "Save Head" after setting up each Laser Head"
</WRAP>
====3. For L... Yes
{{ :support:factory_settings1.png?300 |}}
* After clicking "Yes" you would be asked to insert the s
ll end up in dedicated ''.etc'' data containers.
After importing ''Decay_Coumarin_6.phu'', the workspace... ike this:
{{ :howto:et2_workspace1.png |}}
\\
\\
After expanding the data container:
{{ :howto:et2_works